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Supernatants may be isolated by centrifugation at appropriate speed and duration (e.g., 13,000 rpm for 1 min, 500 rpm for 5 min, etc.)

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By contrast, neutrophils lacking TMEM16F displayed normal NET formation upon PMA stimulation, indicating that TMEM16F, a Ca 2+ -activated scramblase, is dispensable for PMA-induced lipid scrambling and NET formation (Extended Data Fig