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Pyruvate is used by cells in several ways: used in the citric acid cycle to produce energy converted back to glucose via gluconeogenesis converted to lactic acid during fermentation or when oxygen is lacking The reaction facilitated by pyruvate dehydrogenase is: Pyruvate + CoA + NAD+ → Acetyl-CoA + CO2 + NADH This reaction is vital for several reasons: Energy production: Acetyl-CoA enters the citric acid cycle, leading to the production of NADH and FADH₂, which are essential for ATP production via oxidative phosphorylation

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