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Investigators have linked its activity to the promotion of fibroblast outgrowth and migration via the FAK–paxillin pathway, upregulation of the growth hormone receptor in tendon fibroblasts, and a strong angiogenic response mediated in part through VEGFR2 and the nitric oxide system

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To increase activity, we transitioned to PACE using a more stringent circuit, replacing the T7 RNAP promoter with the weaker T3′ RNAP promoter 50 and adjusting RBS strength in front of gIII to reduce transcriptional activation following RNAP liberation (Extended Data Fig