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In terms of endosomal escape, exploitation of “proton sponge effect,” N-terminal stearylation, conjugation of endosomolytic compound, peptides or endosomal escape domains (EEDs) have been studied (Szabó et al., 2022)

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Mice were housed in a temperature-controlled room with a 12-h light/dark cycle, 40%–60% humidity, and ad libitum access to water and food