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Neurological Function Score (Modified Longa System) Neurological deficits were evaluated 1 hour after ET-1 injection using the modified Longa scoring system: • 0: No observable neurological deficit (excluded from analysis) • 1: Failure to fully extend the left forepaw • 2: Consistent circling to the left • 3: Falling or leaning to the left side when ambulating • 4: No spontaneous movement (severe infarction) Animals scoring between 1 and 3 were considered to have moderate ischemic deficits and were included in further analyses