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2.10 Inflammatory cytokines assessment Levels of tumor necrosis factor-alpha in kidney tissue homogenates were quantified using commercial ELISA kits (TNF- α ), IL-1β, and IL-6 (Elabscience Biotechnology Inc., Houston, TX, USA), according to the manufacturer’s instructions

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2.1.2 Mechanisms linking decreased NAD + levels to MetS Decreased NAD + levels are associated with obesity, with studies demonstrating reduced NAD + levels in obese individuals