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Stromal vascular fraction isolation For stromal vascular fraction (SVF) isolation, harvested sWAT depots from male C57Bl/6J mice were finely minced and digested in collagenase solution (2 mg/ml collagenase Type II, 10 mM CaCl2 and 0.5% free fatty acid free BSA in PBS) for 30 min at 37 °C at 16 × g

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Given that research on this compound is still ongoing, ensuring purity and proper formulation is critical to minimizing risks