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How to choose between affinity tags for protein purification | Biotage

The principle of the assay resembles that of affinity purification of proteins in biochemistry: metals binding to the ligand on the beads and the rest in the buffer are separated by a spin-column and quantified by ICP-OES

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Interestingly, an electrophilic molecule, 15-deoxy-∆12,14)-prostaglandin J2, is produced by convoluted blood flow with shear stress and activates Nrf2 in endothelial cells [23]