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The disulfide bridge between Cys₇ and Cys₁₄ is essential for maintaining the peptide’s bioactive conformation

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Antisera against BBK32 C1/C1r domain (33) and VlsE C6 peptide (101) were generated by immunizing Sprague-Dawley rats (Envigo RMS Inc.) with the corresponding purified, recombinant His-tagged protein, as previously described (102)