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Subsequently, 500 μ L of ChIP dilution buffer (10 mm Tris–HCl, pH 8, 150 mm NaCl, 1 mm EDTA, 1.1% Triton X-100, and cOmplete mini protease inhibitor-Roche 11836153001) was added to the lysate, and the mixture was centrifuged at 12,000 g for 10 min at 4 °C

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