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For peptide pull-down of 6xHis-Aurora A, 1 μg Bora (49-69) peptides (WT or S59ph) were incubated with 4 μg of recombinant proteins in 500 μl P150 buffer for 1 h at 4 °C, followed by incubation with NeutrAvidin Plus Ultralink Resin (Thermo Fisher Scientific) for 1 h

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Valproic acid and topiramate induced hyperammonemic encephalopathy in a patient with normal serum carnitine