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Experiments were performed at 37 °C in respiration medium containing EGTA (0.5 mM), MgCl 2 (3 mM), K-lactobionate (60 mM), taurine (20 mM), KH 2 PO 4 (10 mM), HEPES (20 mM), sucrose (110 mM), and BSA (1 g/l), with the addition of mitochondrial substrates and inhibitors (Substrates-Uncouplers- and Inhibitors (SUIT) Protocol) to measure coupled and uncoupled respiration, flux through complex I and II, maximal respiration of the ETS, and cytochrome oxidase (complex IV respiration)

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This link between NNMT and stem cells makes development of therapeutics to treat regenerative-related diseases a relevant target