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The pH of GI tract varies from 1– 7, with stomach pH between 1–3, duodenum pH between 6.0–6.5, and large intestine pH from 5.5–7.0 (Van de Graaff, 1986)

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These intermediates are stabilized through H-bonds between their ribosyl 2′-, 3′- OH groups and the catalytic residue Glu226, a residue required for the NADase and cyclase activity of the enzyme [38-40, 58-60]