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MG-H1 directed search A methylglyoxal hydroimidazolone 1 (MG-H1) directed search was carried out according to the default parameters for a closed search in MSFragger with the following adaptations: precursor match tolerance was set to ± 20 ppm, strict trypsin was used as enzyme but since MG-H1 is expected to be on arginine affecting tryptic cleavage efficiency, missed cleavage allowance was increased from two to three

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[Google Scholar] 264.De la Torre R., Casado A., López-Fernández E., Carrascosa D., Ramírez V., Sáez J