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The evolution of FolEIB in bacteria may be reflective of a GCYH that evolved under the influence of dual stressors, oxidative/nitrosative stress and Zn II limitation, reminiscent of DskA2 vs DksA (Section 4.2.2)

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Phenol:chlorophorm:isoamyl alcohol (25:24:1, v/v/v) was added to the plant extract in a 1:1 ratio, and total DNA was extracted into the aqueous phase by careful mixing

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