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After blocking with blocking solution (0.2% casein, 0.3% Triton X-100, and 3% goat serum diluted in PBS) for 2 h at room temperature, anti-C3 (1:100 dilution) antibody-stained sections were reacted overnight at 4 °C

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Lab studies show that selenium supplements improve sperm viability and mitochondrial membrane potential while reducing malondialdehyde (MDA) levels—a marker of lipid peroxidation [12]