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Similar to total serum copper, in several conditions, such as autoimmune hepatitis, cholestasis syndromes and acute liver failure, urinary copper is elevated as a compensatory mechanism to altered biliary excretion

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After the click reaction, coverslips were washed three times with 1X PBS and incubated with blocking buffer [BSA (3%) + Triton-X 100 (0.1%) in 1X PBS] for 1 h at room temperature